K.K., A.M.W. K.Z., Z.S., S.L.

Cryo-EM structure of a 40-kDa SAM-IV riboswitch RNA at 3.7 Å resolution. In the meantime, to ensure continued support, we are displaying the site without styles You can also search for this author in The ligand-free, closed conformation reveals well-ordered lipids interacting with the channel and two physical constrictions along the ion-conduction pore involving both the extracellular selectivity filter and intracellular helix bundle crossing. You can also search for this author in Huang, L. L., Serganov, A.

Ames, T. D. & Breaker, R. R. Bacterial aptamers that selectively bind glutamine.

K.K.

), the National Science Foundation (GRFP to K.K.

Documentation is available at The links to the EMDB and PDB accession codes in the Data Availability section have been corrected in the HTML and PDF versions of the article.Atkins, J. F., Gesteland, R. F. & Cech, T. RNA Worlds: Cech, T. R. & Steitz, J. developed Eterna3D. performed modeling for all other RNA systems. Meyer, M. et al.

EMDB accession codes are about to change! and JavaScript.The discovery and design of biologically important RNA molecules is outpacing three-dimensional structural characterization. You can also search for this author in

EMDB. Golden, B. L., Gooding, A. R., Podell, E. R. & Cech, T. R. A preorganized active site in the crystal structure of the Marz, M. et al.

and K.K. Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Jaeger, L., Wright, M. C. & Joyce, G. F. A complex ligase ribozyme evolved in vitro from a group I ribozyme domain. A. This work was supported by a Gabilan Stanford Graduate Fellowship (K.K. I.N.Z. This hybrid ‘Ribosolve’ pipeline detects and falsifies homologies and conformational rearrangements in 11 previously unknown 119- to 338-nucleotide protein-free RNA structures: full-length Get time limited or full article access on ReadCube.Cryo-EM maps are available in the EMDB with accession codes The auto-DRRAFTER software is freely available to academic users as part of the Rosetta software package.

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. & Strobel, S. A. The noncoding RNA revolution—trashing old rules to forge new ones. & Serganov, A. De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes. McMullan, G., Faruqi, A. R., Clare, D. & Henderson, R. Comparison of optimal performance at 300 keV of three direct electron detectors for use in low dose electron microscopy.

K.K.

Trausch, J. J. et al. Bergfors, T. Screening and optimization methods for nonautomated crystallization laboratories. UCSF chimera—a visualization system for exploratory research and analysis. ).Present address: The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Sichuan, ChinaPresent address: Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USAThese authors contributed equally: Kalli Kappel, Kaiming Zhang, Zhaoming Su.Biophysics Program, Stanford University, Stanford, CA, USAKalli Kappel, Ved V. Topkar, Ramya Rangan, Wah Chiu & Rhiju DasDepartment of Bioengineering, James H. Clark Center, Stanford University, Stanford, CA, USAKaiming Zhang, Zhaoming Su, Shanshan Li, Grigore Pintilie & Wah ChiuDepartment of Biochemistry, Stanford University, Stanford, CA, USAAndrew M. Watkins, Wipapat Kladwang, Ivan N. Zheludev, Joseph D. Yesselman & Rhiju DasDivision of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Stanford University, Menlo Park, CA, USADepartment of Physics, Stanford University, Stanford, CA, USAYou can also search for this author in Kempf, G., Wild, K. & Sinning, I. 1–8, Tables 1–4, 6 and 7, and captions for Supplementary Table 5 and Supplementary Video 1.RNA structures determined by the Ribosolve pipeline.Auto-DRRAFTER models built using the fully automated procedure.

Tian, S., Kladwang, W. & Das, R. Allosteric mechanism of the Zhao, D. Q.

You can also search for this author in Calculations were performed on the Stanford Sherlock cluster.

Putting the pieces together: integrative modeling platform software for structure determination of macromolecular assemblies. You can also search for this author in Kucukelbir, A., Sigworth, F. J.

Consistent global structures of complex RNA states through multidimensional chemical mapping. Russel, D. et al. Kutchko, K. M. et al.

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